منابع مشابه
Measurement of human growth hormone using a chemiluminescence assay and a "glow" luminometer.
The gene that codes for human growth hormone (hGH) is used as a reporter gene to assess changes an effector may have on gene expression of a specific DNA sequence (6). hGH is secreted into the medium of transfected cells following the addition of an appropriate effector; measurement of hGH levels allows for quantification of the degree of activation (or suppression) of gene expression relative ...
متن کاملA Portable Luminometer with a Disposable Electrochemiluminescent Biosensor for Lactate Determination
A hand-held luminometer for measuring electrochemiluminescence (ECL) for lactate determination and based on one-shot biosensors fabricated using screen-printed electrodes is described. The lactate recognition system is based on lactate oxidase and the transduction system consists of electro-oxidation of luminol, with all the reagents immobilized in a Methocel membrane. The membrane composition ...
متن کاملImmunochemiluminometric assays based on acridinium labels with a microtiter plate luminometer.
Inununoassays based on chemilunlinescent acridinium salts are highlysensitive, robust, and simple (1) but have not been demonstrated in a microplate format because of the lack of suitable luminometers with “on-board” reagent injection systems necessary for quantifying rapidchemiluminescent reactions. We report our initial studies on a two-site immunochemiluminometric assay (IcMA) for thyrotropi...
متن کاملIntegrated luminometer for the determination of trace metals in seawater using fluorescence, phosphorescence and chemiluminescence detection
The paper describes an integrated luminometer able to perform fluorescence (FL), room temperature phosphorescence (RTP) and chemiluminescence (CL) measurements on seawater samples. The technical details of the instrumentation are presented together with flow injection (FI) manifolds for the determination of cadmium and zinc (by FL), lead (RTP) and cobalt (CL). The analytical figures of merit ar...
متن کاملDevelopment of a chemiluminescence competitive PCR for the detection and quantification of parvovirus B19 DNA using a microplate luminometer.
BACKGROUND Quantitative PCR of viral nucleic acids can be useful clinically in diagnosis, risk assessment, and monitoring of antiviral therapy. We wished to develop a chemiluminescence competitive PCR (cPCR) for parvovirus B19. METHODS Parvovirus DNA target sequences and competitor sequences were coamplified and directly labeled. Amplified products were then separately hybridized by specific ...
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ژورنال
عنوان ژورنال: Microchemical Journal
سال: 2013
ISSN: 0026-265X
DOI: 10.1016/j.microc.2012.10.009